Martin Zaback, Mohamed H. Mousa from @temple.edu & Robyn L. Mildren present this #JournalClub "Shining light on #spinal co-ordination: internal states shape #interneuron recruitment of muscle synergies" based on the work of He et al. (2025) ✍️
🔗 physoc.onlinelibrary.wiley.com/doi/10.1113/...
Enduring reduction of resting state functional connectivity (FC) of parvalbumin-positive interneurons (PV-INs) after stroke. Top left: Experimental timeline, with the cranial optical window implanted one week before the first imaging session. Imaging time point at −1 (PRE), 2, 5, 8, 14, 21, and 28 days after stroke. Top right: Left, representative image sequence of cortical PV-IN activity before stroke. The black dot indicates bregma. L: lateral; M: medial; R: rostral; C: caudal (Scale bar, 1 mm). Right, wide-field calcium imaging field-of-view aligned with the surface of the Allen Institute Mouse Brain atlas. The green area on the left hemisphere locates the damaged region. Yellow squares represent cortical areas defined in both left (L, contralesional) and right (R, ipsilesional) hemispheres. Red dot indicates bregma (Scale bar, 1 mm). Middle row: Pairwise Pearson’s correlation coefficients of cortical activity were visualized as averaged correlation matrices for each imaging time point after hemodynamic correction. Bottom row: Network diagrams of statistically significant FC alterations after 2, 5, 8, 14, 21, or 28 days from injury. Blue and red lines denote significant hyper-correlation and hypo-correlation compared to prestroke values, respectively.
How to restore #motor function after #stroke? This study shows in mice that a combination of #RoboticRehabilitation & non-invasive gamma band #neuromodulation improves motor recovery by restoring movement-related oscillations & parvalbumin #interneuron dynamics @plosbiology.org 🧪 plos.io/4n7QJng
Enduring reduction of resting state functional connectivity (FC) of parvalbumin-positive interneurons (PV-INs) after stroke. Top left: Experimental timeline, with the cranial optical window implanted one week before the first imaging session. Imaging time point at −1 (PRE), 2, 5, 8, 14, 21, and 28 days after stroke. Top right: Left, representative image sequence of cortical PV-IN activity before stroke. The black dot indicates bregma. L: lateral; M: medial; R: rostral; C: caudal (Scale bar, 1 mm). Right, wide-field calcium imaging field-of-view aligned with the surface of the Allen Institute Mouse Brain atlas. The green area on the left hemisphere locates the damaged region. Yellow squares represent cortical areas defined in both left (L, contralesional) and right (R, ipsilesional) hemispheres. Red dot indicates bregma (Scale bar, 1 mm). Middle row: Pairwise Pearson’s correlation coefficients of cortical activity were visualized as averaged correlation matrices for each imaging time point after hemodynamic correction. Bottom row: Network diagrams of statistically significant FC alterations after 2, 5, 8, 14, 21, or 28 days from injury. Blue and red lines denote significant hyper-correlation and hypo-correlation compared to prestroke values, respectively.
How to restore #motor function after #stroke? This study shows in mice that a combination of #RoboticRehabilitation & non-invasive gamma band #neuromodulation improves motor recovery by restoring movement-related oscillations & parvalbumin #interneuron dynamics @plosbiology.org 🧪 plos.io/4n7QJng
Enduring reduction of resting state functional connectivity (FC) of parvalbumin-positive interneurons (PV-INs) after stroke. Top left: Experimental timeline, with the cranial optical window implanted one week before the first imaging session. Imaging time point at −1 (PRE), 2, 5, 8, 14, 21, and 28 days after stroke. Top right: Left, representative image sequence of cortical PV-IN activity before stroke. The black dot indicates bregma. L: lateral; M: medial; R: rostral; C: caudal (Scale bar, 1 mm). Right, wide-field calcium imaging field-of-view aligned with the surface of the Allen Institute Mouse Brain atlas. The green area on the left hemisphere locates the damaged region. Yellow squares represent cortical areas defined in both left (L, contralesional) and right (R, ipsilesional) hemispheres. Red dot indicates bregma (Scale bar, 1 mm). Middle row: Pairwise Pearson’s correlation coefficients of cortical activity were visualized as averaged correlation matrices for each imaging time point after hemodynamic correction. Bottom row: Network diagrams of statistically significant FC alterations after 2, 5, 8, 14, 21, or 28 days from injury. Blue and red lines denote significant hyper-correlation and hypo-correlation compared to prestroke values, respectively.
How to restore #motor function after #stroke? This study shows in mice that a combination of #RoboticRehabilitation & non-invasive gamma band #neuromodulation improves motor recovery by restoring movement-related oscillations & parvalbumin #interneuron dynamics @plosbiology.org 🧪 plos.io/4n7QJng
Left: An example image of a filled PV interneuron showing putative autapses with white arrows. Note that all autapses are present in close distance to the soma. Right: Pie charts depicting the percentage of cells showing (1) both structural and physiological autapses, (2) no structural nor physiological autapses, and (3) only structural autapses. Note that only mice that underwent juvenile demyelination have PV interneurons which show structural autapses but no autaptic response.
What role does #myelination play in #PrefrontalCortex development? This study shows that juvenile #demyelination disrupts PV #interneuron firing & self-inhibition, revealing a developmental window where myelination is essential for long-term cortical function @plosbiology.org 🧪 plos.io/4mI4lVO
Left: An example image of a filled PV interneuron showing putative autapses with white arrows. Note that all autapses are present in close distance to the soma. Right: Pie charts depicting the percentage of cells showing (1) both structural and physiological autapses, (2) no structural nor physiological autapses, and (3) only structural autapses. Note that only mice that underwent juvenile demyelination have PV interneurons which show structural autapses but no autaptic response.
What role does #myelination play in #PrefrontalCortex development? This study shows that juvenile #demyelination disrupts PV #interneuron firing & self-inhibition, revealing a developmental window where myelination is essential for long-term cortical function @plosbiology.org 🧪 plos.io/4mI4lVO
Left: An example image of a filled PV interneuron showing putative autapses with white arrows. Note that all autapses are present in close distance to the soma. Right: Pie charts depicting the percentage of cells showing (1) both structural and physiological autapses, (2) no structural nor physiological autapses, and (3) only structural autapses. Note that only mice that underwent juvenile demyelination have PV interneurons which show structural autapses but no autaptic response.
What role does #myelination play in #PrefrontalCortex development? This study shows that juvenile #demyelination disrupts PV #interneuron firing & self-inhibition, revealing a developmental window where myelination is essential for long-term cortical function @plosbiology.org 🧪 plos.io/4mI4lVO
Cooperative actions of #interneuron families support the #hippocampal spatial #code
www.science.org/doi/10.1126/...
This study by Chad R. Camp of cupharmacology.bsky.social et al. investigates selective enhancement of the #interneuron network and gamma-band power via GluN2C/GluN2D NMDA #receptor potentiation 🧠 🔬
📜 Read the #Research here: physoc.onlinelibrary.wiley.com/doi/10.1113/...
Intersectional genetics was used to stochastically label NB5-2 Hb+ neurons. Top row and bottom left show single-cell morphology for the indicated neurons in L1 (0–3 h ALH) larvae. In each set of panels, the top panel shows the TEM reconstructions. Dotted line marks VNC border; gray surface, neuropil. The bottom panels show MCFO single neuron labeling of the indicated neurons. Dotted lines mark the VNC and the neuropil, inset shows Hb expression in each neuron. The bottom right panel shows TEM reconstruction (top panels) and Hb+ NB5-2 lineage labeling (bottom panels) of all Hb+ NB5-2 neurons.
How are the different aspects of interneuron development coordinated? This study shows that the #Drosophila temporal TF Hunchback promotes #interneuron molecular identity, morphology & presynapse targeting in the NB5-2 neural progenitor lineage @plosbiology.org 🧪 plos.io/3Rt2d7d
Intersectional genetics was used to stochastically label NB5-2 Hb+ neurons. Top row and bottom left show single-cell morphology for the indicated neurons in L1 (0–3 h ALH) larvae. In each set of panels, the top panel shows the TEM reconstructions. Dotted line marks VNC border; gray surface, neuropil. The bottom panels show MCFO single neuron labeling of the indicated neurons. Dotted lines mark the VNC and the neuropil, inset shows Hb expression in each neuron. The bottom right panel shows TEM reconstruction (top panels) and Hb+ NB5-2 lineage labeling (bottom panels) of all Hb+ NB5-2 neurons.
How are the different aspects of interneuron development coordinated? This study shows that the #Drosophila temporal TF Hunchback promotes #interneuron molecular identity, morphology & presynapse targeting in the NB5-2 neural progenitor lineage @plosbiology.org 🧪 plos.io/3Rt2d7d
Intersectional genetics was used to stochastically label NB5-2 Hb+ neurons. Top row and bottom left show single-cell morphology for the indicated neurons in L1 (0–3 h ALH) larvae. In each set of panels, the top panel shows the TEM reconstructions. Dotted line marks VNC border; gray surface, neuropil. The bottom panels show MCFO single neuron labeling of the indicated neurons. Dotted lines mark the VNC and the neuropil, inset shows Hb expression in each neuron. The bottom right panel shows TEM reconstruction (top panels) and Hb+ NB5-2 lineage labeling (bottom panels) of all Hb+ NB5-2 neurons.
How are the different aspects of interneuron development coordinated? This study shows that the #Drosophila temporal TF Hunchback promotes #interneuron molecular identity, morphology & presynapse targeting in the NB5-2 neural progenitor lineage @plosbiology.org 🧪 plos.io/3Rt2d7d
Another beauty that I came across during my #2-photon experiments is a Polyaxonal amacrine cell (PAC), a type of #interneuron in the #retina with multiple axon-like processes, said to play a crucial role in visual signal processing and modulation, particularly in the #IPL.
Interested in #perineuronalnets #brain #extracellularmatrix #interneuron #psychiatry please check our review! doi.org/10.1177/107385…
Here we show how #microglia contact #pericytes and #endothelialcells in the #venule end of the #capillary bed. Note also how a #primarycilium of an #interneuron invaginates the microglia. But why? 🤔 journals.sagepub.com/doi/full/10.... @gazz4science.bsky.social
